This volume provides key molecular biological prools and experimental strategies currently employed to study the various stages of mammary gland development. Chapters are divided into four parts covering genetically modified mouse models that exhibit prenatal and pubertal mammary gland phenotypes, 2D and 3D—model culture systems, methodologies for mammary stem cell isolation, and translational applications that provide a bridge between experimental studies of mammary gland development and the study of human breast cancer. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step—by—step, readily reproducible laboratory prools, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting—edge, Mammary Gland Development: Methods and Prools will be useful for researchers whose primary interest is in mammary gland development; developmental biologists, epithelial cell biologists, and those with an interest in molecular mechanisms underlying breast cancer.