During recent years enzyme hishemical reactions have increasingly been considered as important, the reason being that enzyme histo­ chemistry is now a well-established link between morphology and bio­ chemistry. The development of numerous new methods and in particular the improvement of existing techniques contributed to the expansion of enzyme hishemical reactions. Today, the use of these methods allows detailed insight into molecular processes of single cells and their constituents. The selection of a suitable method for enzyme hishemical investigations needs thorough knowledge and critical evaluation of the reactions de­ scribed for the hishemical demonstration of enzymes and introduced in laboratory practice. Often, it is difficult for scientists primarily concerned with the application of methods and for laboratory assistants to comment on the value of an enzyme hishemical reaction. Our book will serve as a guide in this respect. It contains the most important hishemical methods for the localization of enzymes, all of which were checked by the authors themselves. These methods were often modified and frequently used for numerous different investigations of healthy and diseased organs in basic research and in routine practice.