This second edition volume provides an overview of some of the types of nanostructures commonly used in nanobiomedicine. The chapters in this book discuss practical information on the synthesis and characterization of a variety of solution—phase and surface—bound nanomaterials, with examples of how they can be used in sensing, imaging, and therapeutics. Specific topics include the synthesis and characterization of molecule and biomolecule—functionalized nanoconjugates with gold, iron oxide, or polymeric cores; the development of biosensing, imaging, and therapeutic applications of multicomponent/multifunctional nanostructures; and the application of flow cytometry in nanobiomedicine. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step—by—step, readily reproducible laboratory prools, and tips on troubleshooting and avoiding known pitfalls. Thorough and comprehensive, Biomedical Nanotechnology: Methods and Prools, Second Edition is a useful resource for scientists and researchers at all levels who are interested in working in a new area of nanoscience and technology, or in expanding their knowledge base in their current field.