This latest addition to the Methods in Molecular Medicine series, Anti- ral Methods and Prools, is opportune because there is an increasing int- est in discovering compounds that are effective against both chronic and acute viral infections. A number of the methods described in the volume are unp- lished and their inclusion indicates the speed at which this field is moving. This volume is not a review but each chapter contains methods validated by the experts who have spent time in developing the prools. The hallmark of this series is the comprehensive way in which the me- ods are described, which includes a list of all the reagents needed for each prool. Of importance is the section on tips and pitfalls that the authors have discovered while developing their prools. The manual itself is designed to be used by researchers in universities and industry who are familiar with a range of biological techniques but who want to set up quickly a novel assay system. We encourage a dialog between readers and authors, which may also result in useful collaborations.