This detailed volume compiles key methods and techniques used to establish some of the structural and functional aspects of Z—form nucleic acids. Beginning with chapters on characterizing Z—DNA, the book continues by exploring structure determination techniques, gene— and genome—targeting methods and genome—wide approaches, Z—RNA study, and much more. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step—by—step and readily reproducible laboratory prools, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Z—DNA: Methods and Prools serves as an ideal guide for students and researchers curious about this unique Z nucleic acid as an area of investigation, aiming to help the field grow, flourish, and unravel the hidden and novel roles of Z. Chapter 1 is available open access under a Creative Commons Attribution 4.0 International License via link.springer.com. This is an open access book.