This edition of "The Biotechnology of Cannabis sativa" is markedly different than the first edition, which I wrote during my early years of graduate school studying plant biotechnology. That edition was also prompted by furious passion and was quickly written. The result was an inspirational novella that was lacking in technical detail. In this edition, the passion has been retained while some sections become quite technical, allowing the readers to immerse themselves in a deeper level of understanding. Molecular genetics, manipulation of cannabinoid production, tissue culture, transgenic systems, molecular details of gene transfer, cannabis genomics, and bioinformatics are only a few of the emerging sciences that have been included in this edition. Although much of the presentation is in a form that should be pleasantly digestible to almost any reader, there are several sections where technical details were necessarily included.