In this work Ambrose covers the doctrine of regeneration in three tiers. First he covers the doctrinal aspects of the words of Christ in John 3:3, “Except a Man be born again, he cannot see the Kingdom of God.” He explains here the order the Holy Spirit sets down in the necessity, generality, manner and issue of the new birth. In the second part, he further explains the doctrine in relationship to how the new birth changes a Christian towards their duty under the Law in mimicking the character of God as a new creation in Christ. In the third part, he demonstrates the practice and behavior of a man in the new birth outlining the soul’s preparation, God’s part and man’s part in the new birth, God’s work on the soul, closing with Christ, and growing in Christ. This is an important treatise on a foundational topic because Ambrose demonstrates how being or becoming religious is not the same as being regenerate.This work is not a scan or facsimile and has been made easy to read with an active table of contents for electronic versions.