Protein Terminal Profiling: Methods and Protocols

This volume presents detailed prools for novel strategies and approaches to improve functional understanding of protein N- and C-terminal biology. addresses topics such as protease specificity profiling, N-terminal acetylation, assays to probe protease activity in cellular systems, protein N- and C-termini on a proteome-wide scale, and biochemical approaches to explain and examine extracellular protease activities. Written in the highly successful series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory prools, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and thorough, is a valuable resource for researchers that focus on biochemistry and cell biology, and those who share a broad interest in protein functionality and protein modifications.