Twenty-seven chapters deal with the regeneration of plants from protoplasts and genetic transformation in various species of Arachis, Buplerum, Capsella, Dendrobium, Dianthus, Diospyros, Fagopyrum, Festuca, Gentiana, Glycyrrhiza, Gossypium, Hemerocallis, Levisticum, Lonicera, Musa, Physalis, Platanus, Prunus, Saposhikovia, Solanum, Spinacia, Trititrigia, Tulipa, including fruits such as apricot, banana, cranberry, pepino, peach, and plum. These studies reflect the far-reaching implications of protoplast technology in genetic engineering of plants. They are of special interest to research scientists, teachers and advanced students in the fields of plant tissue culture, molecular biology, genetic engineering, plant breeding and general biotechnology.