Plant Cell Electroporation And Electrofusion Protocols in Bloomington, MN

Gene transfer is an essential technology for improving our understanding of gene structure and function. Although there are many methods by which DNA may be introduced into cells—including heat and chemical treatments, and microinjection—electroporation has been found to be the most versatile gene transfer technique. Electroporation is effective with a wide variety of cell types, including those that are difficult to transform by other means. For many cell types, electroporation is either the most efficient or the only means known to effect gene transfer. The early and broad success of electric field-medi­ ated DNA transfer soon prompted researchers to investigate electroporation for transferring other types of molecules into cells, in­ cluding RNA, enzymes, antibodies, and analytic dyes. The first section of Plant Cell Electroporation and Electrofusion Prools includes two chapters that serve as a guide to theoretical and practical aspects of electroporation, and will be of particular interest to those developing prools for as yet untested species or cell types, and a third chapter that describes commercially available electroporation instruments. The remaining chapters describe well-tested prools for DNA electrotransfection, electroporation of other biomolecules, or cell electrofusion. These chapters also include brief discussions of alterna­ tives to electric field-based methods, citing the advantages and limita­ tions of the various methods for achieving specific goals.