Peter Eigil Nielsen has assembled a critically evaluated collection of key PNA prools that are either already well established around the world, such as PCR-clamping and in situ hybridization, or that display promise of significant future impact. Basic methods for PNA oligomer synthesis and analysis have also been included. Written by experts with hands-on in the methods they describe, these readily reproducible prools contain detailed step-by-step instructions, tips on avoiding pitfalls and on extending the method to other situations, and introductory material explaining the theory behind the process.