This volume comprehensively covers new technologies and methodologies that have appeared for the study of mouse development- Update of volume 225, Guide to Techniques in Mouse Development, edited by P.M. Wassarman and M.L. DePamphilis and published in 1993- Comprehensively covers:- new techniques for the cryopreservation of gametes and embryos- production of transgenic and null (knockout) animals (use of ES cells)- generation of conditional/inducible mutant animals, use of gene-trap mutagenesis- analysis of allele-specific expression, use of new reporter constructs- humanizing of transgenic animals- transcript profiling of mouse development- imaging of mouse development- rederivation of animals and use of mouse genomics