This book constitutes the revised selected papers of the 43rd International Workshop on Graph-Theoretic Concepts in Computer Science, WG 2017, held in Eindhoven, The Netherlands, in June 2017.The 31 full papers presented in this volume were carefully reviewed and selected from 71 submissions. They cover a wide range of areas, aiming at connecting theory and applications by demonstrating how graph-theoretic concepts can be applied in various areas of computer science. Another focus is on presenting recent results and on identifying and exploring promising directions of future research.