Genetically Incorporated Non-Canonical Amino Acids: Methods and Protocols in Bloomington, MN

This detailed volume explores non-canonical amino acids (ncAAs) through their site-specific incorporation by genetic code expansion (GCE). The collection provides a broad resource of methods for implementing GCE in
E. coli
, mammalian cells, and animals, highlighting specific applications ranging from fluorescence labeling to phoontrol and the study of protein post-translational modification. Written for the highly successful
Methods in Molecular Biology
series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory prools, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical,
Genetically Incorporated Non-Canonical Amino Acids: Methods and Prools
serves as an ideal source of methodologies that can be adapted and extended, migrated to different model systems, and combined in new ways to help explore a wide range of biological questions and to augment industrial and pharmaceutical protein engineering.